Studying Membrane Protein Thermodynamics Using a Steric Trap
نویسندگان
چکیده
منابع مشابه
Method to measure strong protein-protein interactions in lipid bilayers using a steric trap.
Measuring high affinity protein-protein interactions in membranes is extremely challenging because there are limitations to how far the interacting components can be diluted in bilayers. Here we show that a steric trap can be employed for stable membrane interactions. We couple dissociation to a competitive binding event so that dissociation can be driven by increasing the affinity or concentra...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.286